Employing single-molecule optical and AFM imaging and force-spectroscopy procedures, we search for to unravel the mechanistic facets of the structure and dynamics of chromosomes. Our investigate passions contain:
Human Rad51 filaments on double- and solitary-stranded DNA: correlating standard and irregular forms with recombination purpose.
Multicolor imaging of your bacterial nucleoid and mobile division proteins with blue, orange, and around-infrared fluorescent proteins
I can rarely visualize how I, to be a geneticist, would manage to master elementary physics, let alone compete at the top. How did you go about it?
We establish place-of-care diagnostics check of neglected tropical illnesses within useful resource-constrained settings, based on Crspr/Cas9 detecting of pathogen’s DNA in overall body fluids.
Inside the a long time subsequent these discoveries, my study interests shifted to biophysics and nanobiology, And that i grew to become significantly less associated with the development of nanotube electronics. The main target of many researchers in the sphere also shifted to graphene (wherever, funnily ample, the primary graphene ribbons had been described as ‘nanotubes which have been Slice open along their length’) just after the invention of the two-dimensional product in 2004.
We condition microbes into sorts that deviate from their pure phenotype. Specially, I'll present our capability to shape Are living E. coli microbes into novel designs including rectangles, squares, triangles and circles.
Flow-driven rotary motors for instance windmills and h2o wheels drive practical processes in human Modern society. Although examples of this sort of rotary motors also attribute prominently in mobile biology, their artificial design at the nanoscale has remained tough. Listed here we display flow-driven rotary motion of the self-arranged DNA nanostructure that may be docked onto a nanopore in a skinny solid-state membrane. An elastic DNA bundle self-assembles into a chiral conformation on phoretic docking on to the solid-state nanopore, and subsequently shows a sustained unidirectional rotary movement of up to twenty rev s−one.
‘Sure, I have an understanding of folks were being stunned at some time. At the end of the nineties, Once i was in my forties, I had been Professor of Molecular Electronics and I have to admit that it experienced become a bit bored, I didn’t study Substantially anymore. The sector went extra into the applying facet, how you might combine nanotubes into electronic circuits.
Resolving the phase measurement in condensin-driven DNA loop extrusion identifies ATP binding because the stage-producing method
FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in bacterial cell division
2001, discovery of single-electron transistors at room temperature according to nanotubes; realization of very first logic circuits with carbon nanotube units; and discovery on the molecular framework of DNA mend enzymes with AFM
Developments in nanotechnology in the 1990s made developing electronic gadgets from single molecules a chance. Cees Dekker recounts how his group produced a space-temperature transistor depending on just one carbon nanotube.
2004, discovery of recent physics in translocation of DNA by means of nanopores; initial experimental analyze ceesdekker.net of ions conduction in nanofluidic channels; initially electrochemistry with personal single-wall carbon nanotubes; STM detection and control of phonons in carbon nanotubes; initial electrical docking of microtubules on kinesin-coated nanostructures; initially biophysics characterization on the mechanical Qualities of double-stranded RNA; and to start with solitary-molecule study of DNA translocation by a restriction-modification enzyme.